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. 2019 Mar 10;15(5):1010–1019. doi: 10.7150/ijbs.29680

Figure 5.

Figure 5

An AMPK inhibitor blocked the suppressive effect of metformin on the NLRP3 pathway in cardiomyocytes. In HG-induced cardiomyocytes treated with 2 mM metformin or 2 mM metformin and 10 μM compound C, the expression levels of p-AMPK and AMPK (A), NLRP3 (B), caspase-1 (C) and IL-1β (D) were detected by western blotting. The expression levels of GSDMD-N were determined by western blotting (E) and immunofluorescence staining (F). Scale bar, 100 μm. LC3 (G) and mTOR (H) were detected by western blotting. *P<0.05 compared to the control group, #P<0.05 compared to the HG group. &P<0.05 compared to the HG+Met group. n=3.