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. 2019 Apr 21;15(5):1020–1029. doi: 10.7150/ijbs.27825

Figure 1.

Figure 1

Sqstm1 expression was downregulated in cancer-associated fibroblasts (CAFs) of pancreatic cancer. (A) Representative images of immunohistochemistry (IHC) staining for sqstm1 and LC3 in pancreatic cancer tissues. The table shows expression patterns of sqstm1 in tumor and stromal compartment based on IHC staining. Scale bars represent 50 μm. (B) Representative images of double immunofluorescence staining for sqstm1 and α-SMA in pancreatic cancer tissues. Scale bars represent 50 μm. (C) Fluorescence activated cell sorting (FACS) for EpCAM+ and FAP+ cells and qPCR examining sqstm1 mRNA level for sorted cells. * p < 0.05. (D) Examination of sqstm1 mRNA expression by qPCR for pancreatic cell line and cultured primary CAFs line using β-actin and GAPDH as internal control, respectively. (E) Western blot analysis for sqstm1 for pancreatic cell line and cultured primary CAFs line. (F) Representative image of immunofluorescence staining for α-SMA in one cultured CAFs line was showed. Scale bars represent 20 μm. (G) qPCR for sqstm1 in pancreatic stellate cells (PaSCs) following retinoid acid treatment. (H) Western blot analysis for α-SMA in PaSCs following retinoid acid treatment. ** p < 0.01, **** p < 0.0001.