Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Apr 21;15(5):1020–1029. doi: 10.7150/ijbs.27825

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Ivyspring International Publisher

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.

PMC Copyright notice

Sqstm1 regulates ROS balance through KEAP1/NRF2 signaling. (A) ROS measurement via DCFDA probe detection. (B) Examination of IL8, CXCL1, and CXCL2 mRNA level upon NAC treatment in PaSCs. (C) Western blot analysis for KEAP1 and NRF2 in sqstm1-shRNA transfected PaSCs. (D) Immunofluorescence for NRF2 in sqstm1-downregulated PaSCs. Scale bar represents 10 μm. (E) qPCR for Nqo1 between negative control and sqstm1-shRNA transfected PaSCs. (F) Validation of NRF2 expression change upon siRNA transfection in PaSCs by qPCR. (G) β-galactosidase staining for PaSCs upon transfection of NRF2-siRNA. Scale bar represents 20 μm. ^** p < 0.01, ^*** p < 0.001, ^**** p < 0.0001.