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. 2019 Apr 21;15(5):1042–1051. doi: 10.7150/ijbs.31099

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miR-30e-5p overexpression inhibited hypoxia-induced apoptosis in hiPSC-CMs. (A) RT-qPCR analysis of miR-30e-5p levels in hiPSC-CMs with the indicated treatments. (B) Measurement of hiPSC-CM viability using Caspase-3 activity assay after the indicated treatments. (C) Representative flow cytometry analysis of hiPSC-CMs after annexin-V/PI staining. (D) Quantification of cells positive for annexin-V/PI. (E) Western blot analysis of Bax,Bcl-2, and Caspase-3 protein levels. (F) Quantification of the Caspase-3 protein level. (G) Quantification of the Bax/Bcl-2 protein ratio. (n≥3; *P<0.05, **P<0.01, and ***P<0.001).