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Bim is a direct target of miR-30e-5p in hiPSC-CMs. (A) RT-qPCR analysis of Bim levels in hiPSC-CMs. (B) Western blot analysis of Bim protein. (C)Schematic representation of miR-30e-5p target sequences and Bim 3'-UTR luciferase reporter constructs. (D) The relative luciferase activity in luciferase reporter assays. (E) Level of the autophagy-related proteins Beclin-1 and p62 in hiPSC-CMs after 24 h of hypoxia treatment. (n≥3; *P<0.05, **P<0.01, and ***P<0.001).
