Symbol	Description	Value	Reference
kphos,0	Basal Phosphorylation Rate Constant per site when Ftension= 0	1 s−1	This study: matched to experimental detachment data in wild-type cells. See parameter sensitivity analysis Fig. S4D.
λ	Scaling factor that determines the influence of tension on the phosphorylation rate constant	1 pN−1	Free parameter, see sensitivity analysis in Fig. S4A and Fig. 4D.
Ftension	Centromere tension	2.8, 3.3, 44.9 pN	Matched to experimental average tension values for wild-type and tension mutant cells
β	Sensitivity factor that scaled the fraction of phosphorylated sites with the probability of detachment	10−4	This study: matched to experimental detachment data in wild-type cells. See sensitivity analysis Fig. S4B.
Nsites	Total number of phosphorylation Sites in the kinetochore	1–50	Simulation is insensitive to this parameter (Fig. S4); see sensitivity analysis Fig. S4C.
Nphos	Number of phosphorylated sites	1–50	Determined based on phosphorylation event; varies at each time step in simulation
Δtstep	Simulation time step size	0.01 s	N/A
kdephos	Dephosphorylation rate constant	1 s−1	This study: matched to experimental detachment data in wild-type cells
Δtreattach	Time to reattach a detached kinetochore	210+(rand*60) sec; where rand = uniformly distributed random number between 0 and 1	(Kalinina et al., 2013)
tdur	Simulation duration	20 min	Metaphase time in budding yeast. (Pearson et al., 2001)