Table 3.

Enzymatic properties of wild-type enzymes and fusion proteins

Enzyme	Value ± SD
	Km (g L−1#, mM*, μmol L−1″)	kcat (min−1#, S−1*, S−1″)	Specific activity (U mg−1)	t1/2 (min)
PGL	0.27 ± 0.011	12.69 ± 0.4	279.14 ± 3.5	5.3 ± 0.32
S1nv10-PGL	0.47 ± 0.07	65.6 ± 6.8	499.6 ± 8.4	16.4 ± 1.3
LOX	0.057 ± 0.004	24.7 ± 2.97	30.2 ± 1.22	9.7 ± 0.43
S1nv11-LOX	0.052 ± 0.006	81.2 ± 3.6	38.23 ± 1.87	46.8 ± 1.5
ASN	14.2 ± 0.31	49.2 ± 1.9	17.34 ± 0.16	12.3 ± 0.42
S1nv17-ASN	21.4 ± 0.34	68.6 ± 2.5	21.34 ± 0.6	24.4 ± 0.65

Each result was the average value of three parallel experiments. SD is the standard deviation of three experiments

^#, * and " indicated the units for PGL, LOX, and ASN, respectively