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. 2019 May 12;2019:7609765. doi: 10.1155/2019/7609765

Figure 4.

Figure 4

The effects of sAT on the FOXO3a pathway. (a) The phosphorylation of FOXO3a in the nucleus was measured by Western blotting. (b) Ac-FOXO3a was immunoprecipitated using a FOXO3a antibody. (c) The downstream proteins of FOXO3a (CAT, P27KIP1, and BIM) were measured by Western blotting. HT22 cells were transfected with FOXO3a siRNA for 48 h, then treated with sAT (60 μg/mL) for 24 h and I/R for another 9 h. The levels of CAT (d), ROS (e), and MMP (f) in cells were measured using relative measurement kits. The columns and error bars were represented as means ± SD (n = 3). ##P < 0.01 vs. the Con group, ∗∗P < 0.01 vs. the model group, and &&P < 0.01 vs. the scrb group.