Figure 5.

SAT protected mitochondrial function through the PGC-1a pathway. (a) Ac-PGC-1a levels were measured by immunoprecipitation. (b) The protein expression levels of NRF1, TFAM, P-PGC-1a, and PGC-1a were measured by Western blotting. (c) HT22 cells were transfected with PGC-1a siRNA for 48 h, then treated with sAT (60 μg/mL) for 24 h and I/R for another 9 h. The mitochondrial membrane potential was measured by Rh123 staining. The columns and error bars were represented as means ± SD (n = 3). ^##P < 0.01 vs. the Con group, ^∗∗P < 0.01 vs. the model group, ^&&P < 0.01 vs. the scrb group.