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. 2019 May 15;11:1759091419843762. doi: 10.1177/1759091419843762

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© The Author(s) 2019

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Cx36 expression in rat supraoptic nucleus (SON). (A) Representative confocal microscopic images taken from the SON of VF (a) and Lac (b) female rats. From left to the right: Hoechst staining on cell nuclei, and immunostaining of OT-NP, VP-NP, Cx36, and their merges; the red and green arrows point to OT and VP neurons, respectively. Scale bar, 20 μm. (B) Western blots showing the expression of Cx36 proteins in the SON of VF and Lac rats, respectively. Tubulin immunoreactivity was used as loading control. (C) Western blotting bands showing Cx36 (IP)-pulled down OT-NP in VF and Lac female rats. There was a 1.61 ± 0.1 fold higher association of Cx36 with OT in the SON of lactating rats relative (normalized to 1.0) to that in virgin rats (n = 5 in each group; p < .05 by Student’s t test). Cx36 was used as a control for loading.

Cx = connexin; VF = virgin female; Lac = lactating; IgG-HC = heavy chain of a nonspecific immunoglobulin; IP = immunoprecipitated protein; OT-NP = oxytocin-neurophysin; VP-NP = vasopressin-neurophysin.