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. 2019 Apr 10;98(6):689–697. doi: 10.1177/0022034519839438

Figure 4.

Figure 4.

Wnt-responsive stem/progenitor cells are directly responsible for giving rise to new force-induced periodontal ligament (PDL) and alveolar bone. (A) Experiment design. Tamoxifen was delivered to Axin2CreERT2/+; R26RmTmG/+ mice prior to tooth extraction and examined at days 3, 7, 14, and 28. Cells’ response to Wnt signaling were converted from membrane tomato expressing (red) to membrane GFP expressing (green) in the presence of tamoxifen. (B) Quantification of Wnt-responsive cells in the PDL, normalized to the GFP+ve area in the intact group. (C–G) GFP immunostaining in the PDL at time points indicated. (H) Quantification of Wnt-responsive cells in alveolar bone, normalized to the GFP+ve area in the intact group. (I–M) GFP immunostaining in alveolar bone at time points indicated. (N–R) Immunostaining for collagen I at time points indicated. Scale bars = 50 µm. ab, alveolar bone; d, dentin; GFP, green fluorescent protein. This figure is available in color online.