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. 2019 Apr 16;5(5):842–851. doi: 10.1021/acscentsci.9b00124

Figure 2.

Figure 2

Sequence alignment of McrA peptides and HPLC-based kinetic analysis of the MjYcaO thioamidation reaction. (A) Alignment of McrA peptide sequences (11-mer) flanking the site of thioamidation, including sequences of Mj, Mk, and MaMcrA with the MjMcrA numbering code labeled on both the top and bottom. (B) HPLC-based kinetic analysis on the production of thioamidated MjMcrA 11-mer using varied concentrations of MjMcrA 11-mer peptide substrate.