FIG. 5.

CsA addition to prevent opening the mitochondrial permeability transition pore during reperfusion. (A) 1 μM CsA was added to the reperfusion media (“CsA”). Constructs were analyzed for (B, C) cell death as measured by LDH and AK release, (D) cell viability as measured using RealTime-Glo assay, and (E) mitochondrial membrane permeability as measured by JC-1 dye, where increased ratio of emission at 528 and 590 nm is correlated with higher permeability. Data are from four independent experiments and are shown as individual data points with mean ± SD. Statistical analysis was done using ANOVA with post-hoc Tukey's HSD, ^#Indicates statistical significance compared to all other groups, *Indicates significant difference between groups, p < 0.05. (F) Western blot analysis of apoptosis by cleaved to total caspase 3 ratio. Data are from three independent experiments, mean ± SD. Not significant by unpaired t-test, p < 0.05. CsA, cyclosporine A. Color images are available online.