FIG. 6.

Addition of NAC to reduce reperfusion-induced oxidative stress. (A) 5 mM NAC was added to the reperfusion media (“NAC”). Constructs were analyzed for (B) levels of ROS, (C, D) cell death as measured by LDH and AK release, (E) cell viability as measured using RealTime-Glo assay, and (F) mitochondrial membrane permeability as measured by JC-1 dye, where increased ratio of emission at 528 and 590 nm is correlated with higher permeability. Data aggregated from (B) three and (C–F) four independent experiments. Individual data points with mean ± SD. Statistical analysis was done using ANOVA with post-hoc Tukey's HSD, ^#Indicates statistical significance compared to all other groups, *Indicates significant difference between groups, p < 0.05. NAC, N-acetyl-l-cysteine. Color images are available online.