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. Author manuscript; available in PMC: 2020 May 16.
Published in final edited form as: Cell. 2019 Apr 18;177(5):1217–1231.e18. doi: 10.1016/j.cell.2019.03.036
Ingredients Concentration in Medium (g/L)
Resazurin 0.0001
KH2PO4 2
K2HPO4 2
MgCl2•6H2O 0.2
(NH4)2•SO4 5
L-Glycine 0.075
L-Valine 0.117
L-Leucine 0.131
L-Isoleucine 0.131
L-Methionine 0.149
L-Histidine 0.155
L-Arginine 0.174
L-Phenylalanine 0
L-Tyrosine 0.181
L-Tryptophan 0.204
NaHCO3 2.5
Cysteine HCl 0.5
Glucose 3.603
Trace Mineral Supplement(g/L) 10ml
EDTA 0.5
MgSO4•7H2O 3.0
MnSO4•H2O 0.5
NaCl 1.0
FeSO4•7H2O 0.1
Co(NO3)2•6H2O 0.1
CaCl2 (anhydrous) 0.1
ZnSO4•7H2O 0.1
CuSO4•5H2O 0.010
AlK(SO4)2 (anhydrous) 0.010
H3BO3 0.010
Na2MoO4•2H2O 0.010
Na2SeO3 (anhydrous) 0.001
Na2WO4•2H2O 0.010
NiCl2•6H2O 0.020
Vitamin Supplement (mg/L) 10ml
Folic acid 2.0
Pyridoxine hydrochloride 10.0
Riboflavin 5.0
Biotin 2.0
Thiamine 5.0
Nicotinic acid 5.0
Calcium Pantothenate 5.0
Vitamin B12 0.1
p-Aminobenzoic acid 5.0
Thioctic acid 5.0
Monopotassium phosphate 900.0