Figure 1.

Calotropin inhibits cell proliferation in colorectal cancer cells. (A) Cell viability was measured using an MTT assay in HT-29 and HCT116 cells treated with the indicated concentrations of CTP for 24 hrs. (B and C) Cells were treated with the indicated concentrations of CTP for 24 hrs, and the degree to which CTP inhibited cell proliferation was measured using BrdU labeling. (D–F) Cell proliferation was measured by colony formation assay in HT-29 and HCT116 cells treated with the indicated concentrations of CTP and grown for 7 days. (G–I) BALB/c nude mice were inoculated with HT-29 cells and treated with CTP or vehicle. Tumor volumes were measured at indicated time points (G). Tumor weights at time of sacrifice (H). Images of isolated tumors derived from CTP-or vehicle-treated mice (I). **P<0.01, ***P<0.001.

Abbreviations: CTP, calotropin; CrtL, control.