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. 2019 May 23;12:4047–4054. doi: 10.2147/OTT.S200873

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© 2019 Zhou et al.

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YAP is activated in calotropin-treated colorectal cancer cells. (A) Immunoblot analysis of phosphorylation of YAP (S127) in HT-29 and HCT116 cells treated with the indicated concentrations of CTP for 24 hrs. Total YAP expression served as internal controls. (B and C) ImageJ densitometric analysis of the p-YAP/β-actin ratios from immunoblots. (D) HT-29 cells treated with CTP for 24 hrs，and YAP subcellular localization was determined by immunofluorescence staining for endogenous YAP (green) along with DAPI for DNA (blue). (E) Quantitative analysis of YAP nuclear translocation. (F and G) CTGF and CYR61 mRNA expression in cells treated with the indicated concentrations of CTP for 24 hrs were evaluated by RT-qPCR. *P<0.05, **P<0.01, ***P<0.001.

Abbreviations: CTP, calotropin; CrtL, control.