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. 2019 May 23;12:4047–4054. doi: 10.2147/OTT.S200873

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© 2019 Zhou et al.

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Calotropin promotes degradation of LATS1. (A) Immunoblot analysis of LATS1 in HT-29 and HCT1160 cells treated with CTP for 24 hrs. (B) ImageJ densitometric analysis of the LATS1/β-actin ratios from immunoblots. (C) LATS1 mRNA expression in cells treated with the indicated concentrations of CTP for 24 hrs was evaluated by RT-qPCR. (D) Immunoblot analysis of LATS1 in cells treated with CTP for 24 hrs in the presence of cycloheximide (CHX). (E) At each time point, the intensity of LATS1 was normalized to the intensity of β-actin first and then to the value at the 0-hr time point. (F) Immunoblot analysis of LATS1 in cells treated with CTP for 24 hrs in the presence and absence of MG132. (G) ImageJ densitometric analysis of the LATS1/β-actin ratios from immunoblots. **P<0.01, ***P<0.001.

Abbreviation: CTP, calotropin.