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Protein expression, production, and purification of soluble MT1-MMP (sMT1-MMP). (A) SDS-PAGE analysis of purified protein. Protein was run on 12% SDS-PAGE gel under reducing conditions and (B) analyzed by Western blot with anti-MT1-MMP HPX domain mAb. Gel and Western blot analyses showed the proenzyme (65 kDa) and active (57 kDa) forms of sMT1-MMP.
