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Quantitative RT‐PCR for non‐myogenic markers in human embryonic stem cell (hESC)‐EB‐myoblasts. These include the pluripotency markers OCT4 and SOX2, the neuroectoderm marker PAX6, the cardiogenic mesoderm marker GATA4, the hemangiogenic mesoderm marker FLK1, the endothelial marker VECAD, the endoderm marker AFP and the dermomyotome markers ALX4 and TWIST1. **P < 0.01, EB‐myocytes vs EB
