Figure 7.

Quantitative RT‐PCR for myogenic markers in human embryonic stem cell (hESC)‐monolayer‐myotubes. A, Paired box 3, PAX3; B, Paired box 7, PAX7; C, Myogenic differentiation 1, MYOD1; D, Myogenin, MYOG; E, Neural cell adhesion molecule 1, NCAM1; F, Engrailed 1, EN1; G, Skeletal muscle actin alpha 1, ACTA1; H, Myosin heavy chain, MYHC; I, Embryonic myosin heavy chain 3, MYH3; J, Perinatal myosin heavy chain 8, MYH8; K, Adult slow myosin heavy chain 7, MYH7. **P < 0.01, Primary myotubes vs Monolayer‐myotubes