Table 1.

List of qPCR primers for conventional 384‐well plate qPCR

Gene	Orientation	Sequence 5′‐3′
PAX3	FORWARD	CTC CAC GCT CCG GAT AGT TC
	REVERSE	ATC TTG TGG CGG ATG TGG TT
PAX7	FORWARD	CGT GCT CAG AAT CAA GTT CG
	REVERSE	GTC AGG TTC CGA CTC CAC AT
ALX4	FORWARD	ATG AAT GCT GAG ACT TGC GTC
	REVERSE	GGG AAA TGC CCT AAA AGG CG
SOX2	FORWARD	TTG TCG GAG ACG GAG AAG CG
	REVERSE	TGA CCA CCG AAC CCA TGG AG
PAX6	FORWARD	TCT AAT CGA AGG GCC AAA TG
	REVERSE	TGT GAG GGC TGT GTC TGT TC
TWIST1	FORWARD	CTG CAG CAC CGG CAC CGT TT
	REVERSE	CCC AAC GGC TGG ACG CAC AC
FLK1	FORWARD	AGT GAT CGG AAA TGA CAC TGG A
	REVERSE	GCA CAA AGT GAC ACG TTG AGA T
AFP	FORWARD	AGC TTG GTG GTG GAT GAA AC
	REVERSE	CCC TCT TCA GCA AAG CAG AC
GATA4	FORWARD	CTA GAC CGT GGG TTT TGC AT
	REVERSE	TGG GTT AAG TGC CCC TGT AG
VECAD	FORWARD	TGT GAT GTT GGC CGT GTT AT
	REVERSE	CAG CCC AAA GTG TGT GAG AA
MYOD1	FORWARD	CGG CAT GAT GGA CTA CAG C
	REVERSE	CAG GCA GTC TAG GCT CGA C
MYOG	FORWARD	GGG GAA AAC TAC CTG CCT G
	REVERSE	AGG CGC TCG ATG TAC TGG A
EN1	FORWARD	GTGGTCAAGACTGACTCACGC
	REVERSE	GCTTGTCTTCCTTCTCGTTCTT
NCAM1	FORWARD	ATG GAA ACT CTA TTA AAG TGA ACC TG
	REVERSE	TAG ACC TCA TAC TCA GCA TTC CAG T
ACTA1	FORWARD	CGA CAT CAG GAA GGA CCT GTA TGC C
	REVERSE	GGC CTC GTC GTA CTC CTG CTT GG
MYHC	FORWARD	TTC ATT GGG GTC TTG GAC AT
	REVERSE	AAC GTC CAC TCA ATG CCT TC
MYH3	FORWARD	ATT GCT TCG TGG TGG ACT CA
	REVERSE	GGC CAT GTC TTC GAT CCT GTC
MYH8	FORWARD	TAA ACA CAC CTG CCT GAT GC
	REVERSE	TCA GCT TTA ACA GGA AAA TAA ACG
MYH7	FORWARD	TGC CAC ATC TTG ATC TGC TC
	REVERSE	CTC GGC TTC AAG GAA AAT TG
MYH2	FORWARD	CTG ATG CCA TGG AAT GAC TG
	REVERSE	CCC TAT GCT TTA TTT CCT TTG C
OCT4	FORWARD	GAC AGG GGG AGG GGA GGA GCT AGG
	REVERSE	CTT CCC TCC AAC CAG TTG CCC CAA AC
GAPDH	FORWARD	TGG TAT CGT GGA AGG ACT CA
	REVERSE	TTC AGC TCA GGG ATG ACC TT