Fig. 1. miR-140-3p expression according to HSC-T6 cell activation status. Expression of miR-140-5p was measured in HSC-T6 cells by qPCR. (A) Cells were incubated with 5% FBS for 48 h. The control was FBS-free cells. (B) Cells were treated with 10 ng/mL of PDGF-BB (PDGF) for 48 h. The control was no treatment cells. (C) Cells were exposed to TGF-β1 for 48 h. (D) Levels of the marker of hepatic stellate cell activation, α-SMA in TGF-β1 treated cells were detected using western blot assay. The control was no treatment cells. All experiments were performed in triplicate, and ^*p<0.05, ^†p<0.01. PDGF, platelet derived growth factor; α-SMA, α-smooth muscle actin; TGF-β1, transforming growth factor β1.