Fig. 5. miR-140-3p knockdown inactivates the AKT/mTOR signaling pathway, which is abolished by PTEN silencing. Effects of siPTEN on anti-miR-140-3p-inactivated signaling pathway were measured. Expressions of p-AKT and p-mTOR and PTEN were detected using Western blot assay, and the gray intensity was calculated. All experiments were performed in triplicate. *p<0.05. PTEN, phosphatase and tensin homolog deleted on chromosome.
