Fig. 3. Enrichment of miR-218-5p blocked the killing effect of NK cells to LA cells. (A) Altered abundance of miR-218-5p was detected in IL-2-avtivated NK-92 cells with miR-218-5p or NC transfection. (B and C) IFN-γ and TNF-α secretion were examined in NK-92 cells after IL-2 and miR-218-5p treatment. (D) Killing effect of NK-92 to LA cells was evaluated by LDH cytotoxicity assay kit, and the effect of miR-218-5p on killing effect was detected in NK-92 cells transfected with miR-218-5p mimics. ^*p<0.05, IL-2-treated group vs. control group, and IL-2+miR-218-5p group vs. IL-2+NC group. NK, natural killer; LA, lung adenocarcinoma; IL-2, interleukin-2; NC, negative control; IFN-γ, interferon-γ; TNF-α, tumor necrosis factor-α; LDH, lactate dehydrogenase.