Fig. 4. SHMT1 was a target of miR-218-5p. (A) Bioinformatics assay indicated putative binding sites of miR-218-5p and 3′-UTR of SHMT1 by TargetScan. (B and C) Wt or mut of SHMT1-transfected 293T cells were used to investigate luciferase activity by co-transfection of miR-218-5p or anti-miR-218-5p, respectively. (D and E) Effect of miR-218-5p on SHMT1 expression was detected in NK-92 cells at mRNA and protein level. (F and G) Abundances of SHMT1 mRNA and protein were altered in NK-92 cells after anti-miR-218-5p transfection. ^*p<0.05, miR-218-5p group vs. NC group, and anti-miR-218-5p group vs. anti-NC group. SHMT1, serine hydroxymethyl transferase 1; NK, natural killer.