Figure 2.

Effect of hypoxia on VM formation in SACC cell lines. A, Tube‐like structure formation on Matrigel in SACC‐LM and SACC‐83 cells under normoxia. The SACC‐LM showed a stronger ability of VM formation. For quantitative analysis, six nonoverlapping fields were selected from each culture well and the experiment was performed in quadruplicate (*P < 0.05). B, Hypoxia promoted the VM formation of SACC cells. For quantitative analysis, six nonoverlapping fields were selected from each culture well and the experiment was performed in quadruplicate. (*P < 0.05). C, Scanning electron microscopy was performed to show the tubular profiles were hollow and lined by flattened cancer cells after 7 days on the three‐dimensional cultures. The panel on the left was representative image of cross section of tubular structure. The panel on the right was representative image of vertical sectiono. D, The immunofluorescence staining showed that hypoxia promoted VE‐cadherin of SACC cells