Figure 4.

Exogenous VEGFA promoted EMT and stemness of SACC cells in vitro. A, Immunofluorescence staining (magnification, ×100) was performed to assess the role of VEGFA on EMT of SACC‐LM cells. The data showed that VEGFA enhanced the level of N‐cadherin and inhibition the expression of E‐cadherin while bevacizumab resulted in reverse changes. B, Western blot and RT‐PCR (*P < 0.05) were performed to assess the role of VEGFA on EMT of SACC‐LM cells. The data showed that VEGFA enhanced the level of N‐cadherin and inhibition the expression of E‐cadherin while bevacizumab resulted in reverse changes. C, Immunofluorescence staining (magnification, ×100; bars, 100 μm) and RT‐PCR (*P < 0.05) were performed to assess the role of VEGFA on stemness of SACC‐LM cells. The data showed that VEGFA enhanced the levels of CD44 and ALDH1 while bevacizumab resulted in reverse changes. D, Flow cytometry showed that the expression of CD144 had no difference among three groups