Fig. 4.

Astrocytes conditioned in high glucose media and co-cultured with cerebral microvessel endothelial cells resulted in decreased mitogenic activity. Astrocytes conditioned in high glucose media and co-cultured with cerebral microvessel endothelial cells resulted in decreased BrdU levels (Fig. 4A: n = 7, *p < 0.05), decreased scratch recovery assay results (Fig. 4B: Time accounts for 66.13% of the total variance between the two curves at time point 5 hours, where F = 56.01, p value < 0.0001), and slower tube formation compared to astrocytes conditioned to normal glucose media (Fig. 4C: n = 5).