Figure 2.

miR‐664a‐3p facilitates invasion, migration and EMT process in vitro. A,B, Wound‐healing assay was used to determine the migration of GC cells after transfection of miR‐664a‐3p mimic, NC and inhibitor lentivirous, respectively, in SGC7901 and HGC27. C,D, Effects of miR‐664a‐3p alteration on invasion and migration by transwell assay in vitro. E, Effects of miR‐664a‐3p on EMT process analyzed by Gene Set Enrichment Analysis (GSEA). F, The expression of EMT‐associated proteins detected by Western blot when expression of miR‐664a‐3p was altered in SGC7901 and HGC27. G,H, The Immunofluorescence assay of Ecadherin (red) and Vimentin (green) in SGC7901 and HGC27. The nucleus staining with DAPI (blue).*P < 0.05, **P < 0.01, ***P < 0.001. The data expressed as the mean ± SD