Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 28;5:29. doi: 10.1038/s41421-019-0101-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a Dimeric interface II is mediated by residues N166 in the closed state of AimR. Asn166 of each protomer was mutated to Cys for disulfide bond formation. Both residues are labeled red. b Crosslinking of AimR-N166C. Left upper panel: Crosslinking of AimR-N166C with increasing concentrations of o-phenanthroline copper complex (Cu²⁺). The reactants were subjected to nonreducing SDS-PAGE followed by Coomassie blue staining. Right upper panel: Crosslinking of AimR-N166C with increasing concentrations of Cu²⁺ in the presence of DNA. Left lower panel: Crosslinking of AimR-N166C with increasing concentrations of Cu²⁺ in the presence of an unrelated peptide. Right lower panel: Crosslinking of AimR-N166C with increasing concentrations of Cu²⁺ in the presence of the arbitrium peptide. The highest concentration of Cu²⁺ in lane 8 is 1 mM, and the concentration decreased sequentially by a 1:3 gradient from lane 8 to lane 3. The addition of 5 mM DTT effectively broke the disulfide bond (Lane 1)