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. 2019 May 27;9:7906. doi: 10.1038/s41598-019-44025-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of the binding properties of unlabeled ligands as measured by using two different probes at 25 °C. Binding rate constants of unlabeled ligands were determined in radioligand binding studies. A correlation plot is depicted for the logk_on (a) and logk_off (b) as determined from competitive association experiments using either [³H]mepyramine (x-axis) or [³H]levocetirizine (y-axis) as competitive probe. (c) The correlation plot between the affinity calculated from the kinetic binding rate constants (pK_d,kin) and the affinity from competition binding experiments (pK_i) is depicted. Errors represent SEM values. Dashed lines represent a perfect correlation respective to the X-axis values and solid lines represent the linear regression lines.