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. 2019 May 27;9:7866. doi: 10.1038/s41598-019-44256-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of Pep-H loaded AuNPs. (a) Absorption spectra of AuNPs and Pep-H-AuNPs, (b,c) TEM micrographs of AuNPs and Pep-H-AuNPs respectively for which AuNPs were coated on carbon grids and viewed under transmission electron microscope at magnification of 2,50,000x and scale of 100 nm (d) In vitro release profile of Pep-H-AuNPs in simulated intestinal fluid (SIF), simulated gastric fluid (SGF) and phosphate buffer (PB) for which gold nanoparticles (AuNPs/Pep-H-AuNPs) were placed in 1 ml SIF (pH-6.8)/SGF (pH1.2)/phosphate buffer (pH 7.4). After 1 hr, 3 hrs, 5 hrs, 24 hrs and 72 hrs, nanoparticles were pelleted down and the protein content was estimated in supernatants of all time points by Bradford assay (e) Stability of Pep-H-AuNPs as monitored by UV-Vis spectroscopy for any change in spectra of the pellets of Pep-H-AuNPs following incubation in SIF/SGF/PB. Values are expressed as Mean ± S.D. of 3 independent experiments.