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. 2019 May 27;9:7854. doi: 10.1038/s41598-019-44153-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Typical scanning electron microscopy images of the surface of catheters showing multiple features of leucocyte morphology and interaction with the surrounding environment. (A) Low magnification view of the surface of the catheter showing giant leucocytes interspersed with smaller leucocytes. (B) Leucocyte engulfing a Pseudomonas aeruginosa cell in the area highlighted with the interrupted line box; on the top right corner of the picture there is a higher amplification view of this interaction. (C) Leucocyte adhering to the catheter’s surface. (D) A leucocyte phagocytosing a region with biofilm. (E,F) leucocytes interacting on the surface of the catheter (dotted boxes highlight amplified views of these interactions). (G) Large leucocyte engulfing adjacent biofilm. (H) In the central portion of the image there is a large leucocyte extending a pseudopod into adjacent P. aeruginosa. (I) Three leucocytes with multiple vesicles on their surface. Calibration bar = 100 µm (A) 1 µm (B) 10 µm (C to I).