Table 1.
Average values of bacterial counts determined by different methods on the 7th day postoperatively in the different experimental groups. For each rat, the average number of bacteria on the surface of catheters was based on manual counting bacterial cells on 20 SEM fields at 7500X magnification on each of the two catheter segments, or, when only one catheter segment could be retrieved, on counts performed on 40 SEM fields of that catheter segment. A scanning electron microscope JEOL JSM-5410, with acceleration voltage of 0.015-0.030 V, was used for quantification purposes. Values are expressed as average ± standard deviation. CFU, colony forming units; SEM, Scanning electron microscopy.
| Experimental Group | Viable bacteria cultured from skin flap biopsy (CFU/mg) | Real-time PCR from skin flap biopsy (ng/µl) | Bacteria on the surface of the foreign body (n/SEM field) |
|---|---|---|---|
| NaCl | 2.42 × 105 ± 5.87 × 105 | 1.23 × 10−5 ± 2.06 × 10−5 | 7.74 ± 8.18 |
| PA | 6.98 × 106 ± 1.17 × 107 | 1.26 × 10−1 ± 1.17 × 107 | 71.70 ± 47.4 |
| GFP | 9.46 × 105 ± 6.46 × 105 | 7.22 × 100 ± 1.36 × 101 | 92.75 ± 35.9 |
| BD-2 | 8.06 × 105 ± 8.59 × 105 | 5.52 × 10−1 ± 7.97 × 10−1 | 67.08 ± 42.4 |
| BD-3 | 2.51 × 105 ± 5.88 × 105 | 1.13 × 10−5 ± 1.39 × 10−5 | 19.37 ± 16.3 |
| BD-2 + BD-3 | 3.55 × 105 ± 4.52 × 105 | 1.94 × 10−3 ± 2.92 × 10−3 | 54.04 ± 41.6 |
| Statistical analysis summary | No significant differences were found | No significant differences were found | NaCl < PA; p = 0.013 |
| NaCl < GFP; p < 0.001 | |||
| NaCl < BD-2; p = 0.010 | |||
| PA > BD-3; p = 0.042 | |||
| GFP > BD-3; p < 0.001 | |||
| BD-2 > BD-3; p = 0.035 |