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. 2019 May 27;9:7867. doi: 10.1038/s41598-019-44331-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic comparison of diffraction-limited microscopy and true nanoscale imaging via dSTORM. (a) Schematic diagram demonstrates the difference in spatial resolution between diffraction-limited microscopy and dSTORM imaging. A comparison between diffraction-limited microscopy and dSTORM imaging of our isolated adult mouse ventricular myocytes stained for DHPR, RyR2, PLN, and SERCA2A shows true molecular scale visualization of protein cluster properties. (b) Immunoblot analysis of DHPR, RyR2, PLN, and SERCA2A in isolated adult cardiomyocytes show the detection of their expected molecular weight bands.