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. 2019 May 27;12:37. doi: 10.1186/s12284-019-0297-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 5 — OsPP2C50 and OsPP2C53 physically interact with SAPK10 and their overexpression promotes water loss. a BiFC analysis of VN-SAPK10 and OsPP2C53-VC or OsPP2C50-VC. OsSar1p-VC was used as a negative control. Scale bars are 10 μm. b Co-IP analysis using OsPP2C53-GFP or OsPP2C50-GFP and SAPK10-Flag expressed in rice protoplasts. These experiments were repeated three times and a representative result was presented. c Water loss assays of OsPP2C53-overexpressing transgenic lines (E25–1, 2 and 3) and control line (A8: empty vector transgenic plant). d Water loss assay of OsPP2C50-overexpressing transgenic lines (E40–1, 4 and 5) and control line (A8: empty vector transgenic plant). The values depict the average and ± SD with 3 replications. ANOVA analyses were performed by comparing with water loss of A8 plant.