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. 2019 May 21;10:1079. doi: 10.3389/fimmu.2019.01079

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Copyright © 2019 Shinde, Melinkeri, Santra, Kale and Limaye.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Functional characteristics of HD-SC-DCs and MM SC-DCs were comparable. (A) Percent dextran-FITC uptake by HD-SC-DCs and MM-SC-DCs were similar after 30 and 60 min. (B) Allogeneic T cells co-cultured with both SC-DCs showed similar proliferation, as observed by CFSE dye dilution in proliferating T cells. (C) Secretion of IL-12p70 and (D) IL-10 cytokines in culture supernatants of HD-SC-DCs and MM-SC-DCs showed marginal difference. (E) Concentration of IFNγ in supernatant of SC-DCs and allo-T cell co-cultures were equivalent, as assessed by sandwich ELISA. (F) Migration of SC-DCs toward CCL-19 from HD and MM samples was similar. Expression of CCR7 (G) mRNA and (H) protein was similar in SC-DCs of HD and MM samples, as observed by RT-PCR and FACS, respectively. Data given are mean ± S.E.M.