FIGURE 4.

Overexpression of the malR gene causes global changes in the C. glutamicum transcriptome. Comparative transcriptome analysis of C. glutamicum ATCC 13032 cells carrying the overexpression vector pEKEx2-malR and a strain carrying the empty plasmid. Cells were cultivated in CGXII glucose minimal medium and harvested in the early exponential growth phase. Shown is an MA-plot where the log2 of the expression ratio is plotted against the fluorescence intensity of the single spots. Red dots indicate genes that were bound by MalR in the ChAP-Seq experiment (Figure 2).