Table 2.
Strains and plasmids used in this work.
| Strain or plasmid | Relevant characteristics | Source |
|---|---|---|
| E. coli | ||
| DH5α | supE44ΔlacU169 (φ80lacZDM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Invitrogen |
| BL21 (DE3) | F- ompT hsdSB (rB-, mB-) gal dcm (DE3) | Studier and Moffatt, 1986 |
| C. glutamicum | ||
| ATCC 13032 | Biotin-auxotrophic wild type | Kinoshita et al., 2004 |
| ATCC 13032 ΔmalR | ATCC 13032 with an in-frame deletion of malR (cg3315) | This work |
| ATCC 13032::malR-strep | ATCC 13032 coding for a Strep-Tag fused C-terminally to MalR; integrated at the native locus | This work |
| ATCC 13032::Plys-eyfp | ATCC 13032 containing the prophage reporter Plys-eyfp integrated into the intergenic region of cg1121-cg1122 | Helfrich et al., 2015 |
| Plasmids | ||
| pEKEx2 | KanR; expression vector with lacIq, Ptac and pUC18 multiple cloning site | Eikmanns et al., 1991 |
| pEKEx2-malR | KanR; Ptac, overexpression vector for malR (cg3315) | This study |
| pJC1 | KanR, AmpR, and C. glutamicum shuttle vector | Cremer et al., 1990 |
| pJC1-Plys-‘lys-venus | KanR, AmpR, and prophage reporter plasmid with the gene for the fluorescent protein Venus under control of the promoter of cg1974 (putative phage lysine) | (Pfeifer, unpublished) |
| pK19 mob sacB | KanR; plasmid for allelic exchange in C. glutamicum; (pK18 oriVE.c., sacB, lacZα) | Schäfer et al., 1994 |
| pK19-malR-C-strep | KanR; plasmid for tagging malR (cg3315) genomically with a C-terminal Strep-tag | This study |
| pK19-ΔmalR | KanR; plasmid for the in-frame deletion of malR (cg3315) | This study |
| pET24b | KanR; vector for overexpression of genes in E. coli, with optional C-terminal hexahistidine affinity tag (pBR322 oriVE.c. PT7 lacI) | Novagen |
| pET24b-malR-C-strep | KanR; plasmid for overexpression of C-terminal Strep-tagged malR | This study |