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. 2019 Mar 20;52(3):e12581. doi: 10.1111/cpr.12581

Figure 2.

Figure 2

KK‐LC‐1 knockdown suppresses HCC cell proliferation, migration and invasion in vitro. A, KK‐LC‐1 expression in SMMC7721 and HCCLM3 was stably knocked down using shRNA. RT‐qPCR was performed to assess the knockdown efficiency. B, Western blot was conducted to evaluate the effects of sh‐KK‐LC‐1 on SMMC7721 and HCCLM3 cells. C, CCK‐8 assays were used to analyse cell proliferation in HCC cells with KK‐LC‐1 knockdown. D, EdU assays were performed to examine the effects of KK‐LC‐1 knockdown on HCC cell proliferation ability. Proportion of EdU‐positive cells was determined and statistically analysed. E, Colony formation assays were conducted in sh‐KK‐LC‐1 HCC cells and control cells. Number of colonies was counted and shown as histogram. F, Transwell assays were used to analyse HCC cell motility. Migrated and invaded cells were quantified and statistically analysed. The data are shown as the mean ± SE. *P < 0.05, **P < 0.01, ***P < 0.001.