Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 21;10:610. doi: 10.3389/fpls.2019.00610

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Zhang, Burel, Plasson, Kiefer-Meyer, Ovide, Gügi, Wan, Teo, Mak, Song, Driouich, Lerouge and Bardor.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Immunocytochemical localization of V5-tagged FuT54599 and V5-tagged GnT I in the Golgi apparatus of P. tricornutum. Transmission Electron micrographs of HPF/FS P. tricornutum cells which were embedded in LRW resin. (A) View showing a Golgi apparatus of P. tricornutum oriented as previously described for algae cells in Donohoe et al. (2007, 2013). (B) Localization of the V5-tagged GnT I in the medial-trans Golgi cisternae of P. tricornutum Golgi apparatus after immunolabelling with a mouse anti-V5 antibody used as a primary antibody (dilution 1/20) and a secondary antibody coupled to 10 nm gold beads (dilution 1/20) and then contrasted with uranyl acetate and lead citrate. (C) Localization of the V5-tagged FuT54599 in the median-trans Golgi cisternae of P. tricornutum Golgi apparatus after immunolabelling with a rabbit anti-V5 antibody used as a primary antibody (dilution 1/20) and a secondary antibody coupled to 10 nm gold beads (dilution 1/20) and then contrasted with uranyl acetate and lead citrate. N, nucleus; G, Golgi apparatus with cis and medial/trans cisternae; V, vacuole; m, mitochondria; p, pyrenoid; C, chloroplast; Cw, cell wall. Scale bar: 0.5 μm. Please refer to the Supplementary Figure S4 for negative controls.