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Colony PCR (1% agarose gel) confirming chromosomal integration and segregation of the efe expression cassettes at the NSI-locus of Synechococcus elongatus PCC 7942. Wild type control (WT; expected fragment size = 906 bp), Synechococcus carrying efe from P. Syringae (o-efe; expected fragment size = 5446 bp), and Synechococcus carrying sequence optimized efe (sy-efeh; expected fragment size = 5446 bp). The NSI-specific primers used for the colony PCR are listed in Table 2
