Skip to main content
. 2019 May 21;10:312. doi: 10.3389/fendo.2019.00312

Figure 1.

Figure 1

The close association of kisspeptin and gonadotropin-releasing hormone (GnRH) fibers in the median eminence of female rats. (A) An immunofluorescence photomicrograph showing kisspeptin (red)- and GnRH (green)-immunoreactivities in the arcuate nucleus (ARC)-median eminence (ME) region. Note that the square in the internal zone of the median eminence shows the area for electron microscopy. Scale bar, 50 μm. 3V, third ventricle. (B,C) Immunoelectron micrographs showing kisspeptin- (red asterisks) and GnRH-immunoreactive fibers (green asterisks) visualized by diaminobenzidine and silver enhancement of colloidal gold, respectively. Solid and open arrowheads show kisspeptin- and GnRH-immunoreactive secretory granules, respectively. Open arrows show non-synaptic contact between kisspeptin- and GnRH-neuronal elements. Scale bar, 500 nm. (D) Electron micrograph showing synaptic contacts (solid allows) in other neurons. Scale bar, 500 nm. Originally published in Uenoyama et al. (66) (permission was obtained from John Wiley and Sons).