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. 2019 May 21;9:165. doi: 10.3389/fcimb.2019.00165

Figure 2.

Figure 2

Representative NEC multilayers supported colonization of microbial communities as revealed by SEM imaging at 5–20 k magnification (A–L) and qPCR (M,N). A–B. NEC03 multilayers seeded with NMB from a healthy donor revealed numerous bacterial cell morphologies, predominantly rods and cocci. C–D. NEC03 multilayers seeded with NMB from a donor who recently received antibiotic therapy revealed predominantly clustered cocci, consistent with Staphyloccocus spp. (E–J) NEC03 multilayers seeded with NMB were examined at 4 (E,F), 24 (G,H) and 48 h (I–J). Proliferation and stabilization of bacterial communities were most evident by 24 and 48 h, with extracellular polymeric substances deposition observed at 48 h (I). (K,L). NEC02 multilayers seeded with S. aureus displayed confluent colonization by the bacteria. (M) Health of the NEC02 was maintained in the presence of 7 distinct NMB over 72 h as indicated by genomic counts (hGAPDH). (N) NEC02 multilayers supported bacterial colonization by distinct NMB over time.