Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Strategy for serial genome editing in B. subtilis. The pG2 plasmid containing sgRNAct, sgRNAst, and donor DNA fragment is introduced into pHCas9-containing cells. In the growth phase, sgRNAct/Cas9 complex is responsible for target gene editing. In the sporulation phase, sgRNAst/Cas9 complex make double strand breaks in the replication origin (rep) of the pG2 for self-curing. Thus, the cells are ready for the next round of editing which can be transformed with the new pG2 plasmid.
