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. 2019 May 21;10:1140. doi: 10.3389/fmicb.2019.01140

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Copyright © 2019 Lim and Choi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Plasmid curing efficiency, according to the existence of the sgRNA_st. (A) The construction of two plasmids pG-aE and pG2-aE for aprE gene deletion and self-curing. (B) The comparison of plasmid curing efficiency. The use of IPTG could increase the curing efficiency of pG2-aE from 74 to 98%. The bars display the means of three independent experiments, with the error bars indicating standard deviations.