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The protease assay using FTC-Casein as substrate for confirming the serial deletion of extracellular protease genes. The protease activity of deletion mutants is compared to the wild type strain (B. subtilis 168) and the WB800N. The protease activity was assessed as changes in fluorescence through the digestion of the FTC-Casein substrate. The bars display the means of three independent experiments, with the error bars indicating standard deviations.
