Table 2.
Oligonucleotides and Primers used in this study.
| Oligonucleotide | Sequence (5′→3′) |
|---|---|
| aprE-gF1 | Attgaccgattgagttattaagag |
| aprE-gR1 | Aaacctcttaataactcaatcggt |
| nprE-gF1 | Attgagacaagcgtgcccggaagg |
| nprE-gR1 | Aaacccttccgggcacgcttgtct |
| epr-gF1 | Attgttaaaccagtattatgcaac |
| epr-gR1 | Aaacgttgcataatactggtttaa |
| bpr-gF1 | Attgataacggaaagacatcaagc |
| bpr-gR1 | Aaacgcttgatgtctttccgttat |
| mpr-gF1 | Attgtatccgtacggtacttattc |
| mpr-gR1 | Aaacgaataagtaccgtacggata |
| nprB-gF1 | Attgtctcaactgatcgggtatac |
| nprB-gR1 | Aaacgtatacccgatcagttgaga |
| vpr-gF1 | Attgaaagtcgccgttgtcaaacg |
| vpr-gR1 | Aaaccgtttgacaacggcgacttt |
| wprA-gF1 | Attgatattcagtacccttatcaa |
| wprA-gR1 | Aaacttgataagggtactgaatat |
| Primer | Sequence (5′→3′) |
| spoIVA-rep-F1 | Caagaattcgatgtcatattcaaataggacaacgtcatacacatatagtgca aatttctgcgatgatggagttttagagctagaaatagcaagttaaa |
| spoIVA-rep-R1 | Agaggatccaaaaaaagcaccgactcggtgccactttttcaagttgataac ggactagccttattttaacttgctatttctagctctaaaactccat |
| g-AarI-F1 | Taagatctaagcttaaagattgacagtataatagtc |
| g-AarI-R1 | Aaaatgcatactagtagctagcaggatccaaaaaaagcaccgactc |
| aprE-NF1 | Tggatcctagttatttcgagtctctacgg |
| aprE-NR1 | tgtgcaatatgatcttcttcc |
| aprE-CF1 | Ggaagaagatcatattgcacatcggctaccctgcaaaatat |
| aprE-CR1 | Atactagtcggtgcttgtgaagattttca |
| nprE-NF1 | Tggatccttatcaatcagcctgccaggt |
| nprE-NR1 | Taagcaagacgatagctgcc |
| nprE-CF1 | Ggcagctatcgtcttgcttatgatggcgacggttcattctt |
| nprE-CR1 | Atactagtcttcaactttagcggcatca |
| epr-NF1 | Gcagaccaggagacagtaaaa |
| epr-NR1 | Tcgtgccaaggctcatattga |
| epr-CF1 | Tcaatatgagccttggcacgatgccgaactccgacgccaaaa |
| epr-CR1 | Atactagttcttcactttgtctaaccgtt |
| bpr-NF1 | Tggatccatttcctgattcaccgaataa |
| bpr-NR1 | Cggaactccccattcccagtt |
| bpr-CF1 | Aactgggaatggggagttccgatcagaacaaggctatacag |
| bpr-CR1 | Atactagtataaccgacgaaaggcttcaa |
| mpr-NF1 | Tggatcctgctgctgattcagttgaaa |
| mpr-NR1 | Ggctgtaaggtttgaatgttt |
| mpr-CF1 | Aaacattcaaaccttacagccctgaaacgtataagctgacct |
| mpr-CR1 | Atactagtgagatgtgatgggttactgat |
| nprB-NF1 | Tgctagcatcaaaccccttcatacatca |
| nprB-NR1 | Cagatgtcgagagtctcacaa |
| nprB-CF1 | Ttgtgagactctcgacatctgatgaaatcacacacgcagtca |
| nprB-CR1 | Atactagtatagaatgccgacagcctca |
| vpr-NF1 | Tggatcctctccgcaaatggatgacagt |
| vpr-NR1 | Ctgttgccgtttgaggtaac |
| vpr-CF1 | Gttacctcaaacggcaacagtgttatggatacgtggatgat |
| vpr-CR1 | Atactagttacttttgcagtggctttccc |
| wprA-NF1 | Tggatccggttgaaatgagtgtcgatca |
| wprA-NR1 | Ttatgtacggatgagaggct |
| wprA-CF1 | Agcctctcatccgtacataattgcagcccaaagcgataac |
| wprA-CR1 | Atactagtagcttaggattttgagcaaac |
Underlined sequences are the restriction enzyme sites. Bolded sequences represent the 20 bp synthetic gRNA.