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. 2019 May 21;13:108. doi: 10.3389/fnhum.2019.00108

Figure 1.

Figure 1

Midazolam disturbs lysosomal homeostasis. HEK293 and Hela cells were treated with 15 μM or 30 μM midazolam for 0–36 h. The size and morphology of the lysosomes were observed under a microscope. (A,D) Lysosomal staining pattern of HEK293 cells and HeLa cells treated with 15 μM or 30 μM midazolam at 0 h, 24 h, and 36 h, respectively. (B,E) Statistical results of the average size of lysosomes in HEK293 cells and HeLa cells, respectively. (C,F) Statistical results of the average number of lysosomes per HEK293 and HeLa cell, respectively. For lysosomal number and lysosomal size statistics, at least 30 cells per group were counted. Scale bar = 2 μM (Mean ± SEM. ***p < 0.001, ns, no significant).